Extracellular carbohydrate esterase from the basidiomycete Coprinopsis cinerea released ferulic and acetic acids from xylan

Biosci Biotechnol Biochem. 2010;74(8):1722-4. doi: 10.1271/bbb.100299. Epub 2010 Aug 7.

Abstract

cDNA encoding an extracellular carbohydrate esterase (CcEst1) was cloned from the basidiomycete Coprinopsis cinerea. The recombinant CcEst1 expressed in Pichia pastoris acted on p-nitrophenyl acetate, alpha-naphthyl acetate, and methyl hydroxycinnamic acids, except for methyl sinapic acid. The enzyme released ferulic and acetic acids from wheat arabinoxylan and acetylated xylan respectively. Activity increased on the addition of endo-beta-1,4-xylanase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetic Acid / metabolism*
  • Agaricales / cytology*
  • Agaricales / enzymology*
  • Agaricales / metabolism
  • Amino Acid Sequence
  • Cloning, Molecular
  • Coumaric Acids / metabolism*
  • Esterases / chemistry
  • Esterases / genetics
  • Esterases / metabolism*
  • Extracellular Space / enzymology*
  • Molecular Sequence Data
  • Xylans / metabolism*

Substances

  • Coumaric Acids
  • Xylans
  • ferulic acid
  • Esterases
  • Acetic Acid

Associated data

  • GENBANK/AB540992