Establishment and characterization of a new human leukemia cell line derived from M4E0

Blood. 1991 Jul 15;78(2):451-7.


A new human leukemia cell line, designated as ME-1, was established from the peripheral blood leukemia cells of a patient with acute myelomonocytic leukemia with eosinophilia (M4E0). This cell line has the characteristic chromosome abnormality of M4E0, inv(16) (p13q22). When cultured in RPMI 1640 medium containing 10% fetal calf serum, ME-1 cells were monoblastoid, but with the addition of cytokines such as interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-4, or medium conditioned by phytohemagglutinin-stimulated human peripheral leukocytes (PHA-LCM), the cells exhibited differentiation to macrophage-like cells. PHA-LCM also promoted eosinophilic-lineage differentiation of this cell line, although IL-5 did not do so. To elucidate the mechanism of proliferation and differentiation of ME-1 cells, we studied the effect of a potent inhibitor of protein kinase C, 1-(5-isoquinolinyl-sulfonyl)-2-methylpiperazine (H-7), on colony formation of ME-1 cells. H-7 inhibited colony formation of ME-1 cells by IL-3 or GM-CSF dose dependently, but had little inhibitory effect on colony formation by IL-4. These results indicate that the proliferation and differentiation of ME-1 cells by IL-3 or GM-CSF were related to the activation of protein kinase C, while those by IL-4 involved other regulatory systems. ME-1 cells should be useful for studying the pathogenesis of M4E0 and the mechanisms of proliferation and differentiation of leukemic and normal progenitors by cytokines.

Publication types

  • Case Reports

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Adult
  • Antigens, Surface / analysis
  • Cell Adhesion / drug effects
  • Cell Differentiation / drug effects
  • Cell Line
  • Chromosome Banding
  • Culture Techniques / methods
  • Cytokines / pharmacology
  • Eosinophilia / blood*
  • Eosinophilia / genetics
  • Eosinophilia / pathology
  • Humans
  • Isoquinolines / pharmacology
  • Karyotyping
  • Kinetics
  • Leukemia, Myelomonocytic, Acute / blood*
  • Leukemia, Myelomonocytic, Acute / genetics
  • Leukemia, Myelomonocytic, Acute / pathology
  • Macrophages / ultrastructure
  • Male
  • Microscopy, Electron
  • Muramidase / blood
  • Phagocytosis
  • Piperazines / pharmacology
  • Protein Kinase C / antagonists & inhibitors


  • Antigens, Surface
  • Cytokines
  • Isoquinolines
  • Piperazines
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Protein Kinase C
  • Muramidase