Antimicrobial Susceptibility and Methicillin Resistance in Staphylococcus Pseudintermedius and Staphylococcus Schleiferi Subsp. Coagulans Isolated From Dogs With Pyoderma in Japan

J Vet Med Sci. 2010 Dec;72(12):1615-9. doi: 10.1292/jvms.10-0172. Epub 2010 Aug 10.

Abstract

To understand species distribution, trends of antimicrobial susceptibility and prevalence of methicillin resistance in canine staphylococci in Japan, 190 coagulase-positive staphylococci (CoPS) were isolated from dogs with pyoderma in 2 Japanese veterinary referral hospitals. Using a multiplex polymerase chain reaction (M-PCR) method, two CoPS species were identified: 170 Staphylococcus pseudintermedius (89.5%) and 20 S. schleiferi subsp. coagulans isolates (10.5%). In these isolates, susceptibility to 7 antimicrobial agents was determined. Overall, the levels of susceptibility to cefalexin (CEX), amoxicillin/clavulanic acid (CVA/AMPC), minocycline (MINO), ofloxacin (OFLX), norfloxacin (NFLX), lincomycin (LCM) and clindamycin (CLDM) in S. pseudintermedius isolates were 38.2, 52.4, 34.7, 31.2, 34.1, 1.2 and 11.2%, respectively. In S. schleiferi subsp. coagulans isolates, 55% demonstrated susceptibility to CEX, 80% to CVA/AMPC, 70% to MINO, 45% to OFLX or NFLX and 30% to CLDM. None of S. schleiferi subsp. coagulans isolates was susceptible to LCM. To determine the prevalence of methicillin-resistant strains, we used a PCR method, which enabled detection of the fragment of mecA gene in 66.5% (113 of 170) in S. pseudintermedius and 30.0% (6 of 20) in S. schleiferi subsp. coagulans isolates. The frequencies of susceptibility to CEX, CVA/AMPC, OFLX, NFLX and CLDM were significantly lower in methicillin-resistant CoPS than in methicillin-susceptible CoPS isolates. These data suggest a high level of methicillin resistance in staphylococci isolated from dogs with pyoderma in Japan.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Dog Diseases / epidemiology
  • Dog Diseases / microbiology*
  • Dogs
  • Japan / epidemiology
  • Methicillin Resistance*
  • Pyoderma / epidemiology
  • Pyoderma / microbiology
  • Pyoderma / veterinary*
  • Staphylococcal Skin Infections / microbiology
  • Staphylococcal Skin Infections / veterinary*
  • Staphylococcus / classification
  • Staphylococcus / drug effects*

Substances

  • Anti-Bacterial Agents