We proposed Index-Blocked Discrete Cosine Transform Filtering Method (IB-DCTFM) to design ideal frequency range filter on DCT domain for biomedical signal which frequently exposed to specific frequency noise such as motion artifacts and 50/60 Hz powerline interference. IB-DCTFM removes unwanted frequency range signal on time domain by blocking specific DCT index on DCT domain. In simulation, electrocardiography, electromyography, photoplethysmography are used as a signal source and FIR, IIR and adaptive filter are used for comparison with proposed IB-DCTFM. To evaluate filter performance, we calculated signal-to-noise ratio and correlation coefficient to clean signal of each signal and filtering method respectively. As a result of filter simulation, average signal to noise ration and correlation coefficient of IB-DCTFM are improved about 75.8 dB/0.477, and FIR, IIR and adaptive filtering results are 24.8 dB/0.130, 54.3 dB/0.440 and 29.5 dB/0.200 respectively.