A systems approach to analyze transcription factors in mammalian cells

Methods. 2011 Feb;53(2):151-62. doi: 10.1016/j.ymeth.2010.08.002. Epub 2010 Aug 10.


Transcription factors (TFs) play a central role in the development of multicellular organisms. The sequential actions of critical TFs direct cells to adopt defined differentiation pathways leading to functional, fully differentiated tissues. Here, we describe a generic experimental pipeline that integrates biochemistry, genetics and next generation sequencing with bioinformatics to characterize TF complexes composition, function and target genes at a genome-wide scale. We show an application of this experimental pipeline which aims to unravel the molecular events taking place during hematopoietic cell differentiation.

Publication types

  • Review

MeSH terms

  • Animals
  • Binding Sites
  • Cell Differentiation / genetics*
  • Chromatin Immunoprecipitation
  • Embryo, Mammalian / metabolism
  • Embryo, Nonmammalian / metabolism
  • Gene Expression Regulation
  • Mice
  • Systems Biology / methods*
  • Transcription Factors / physiology*
  • Zebrafish / embryology


  • Transcription Factors