Dietary broccoli sprouts protect against myocardial oxidative damage and cell death during ischemia-reperfusion

Plant Foods Hum Nutr. 2010 Sep;65(3):193-9. doi: 10.1007/s11130-010-0182-4.


Cruciferous vegetables are known for antioxidant and anti-carcinogenic effects. In the current study we asked whether dietary broccoli sprouts can protect the heart from ischemia-reperfusion. Rats were fed either control diet (sham and control groups) or a diet mixed with 2% dried broccoli sprouts for 10 days. After 10 days the isolated hearts were subjected to ischemia for 20 min and reperfusion for 2 h, and evaluated for cell death, oxidative damage, and Nrf2-regulated phase 2 enzyme activities. Broccoli sprouts feeding inhibited markers of necrosis (lactate dehydrogenase release) and apoptosis (caspase-3 activity) by 78-86%, and decreased indices of oxidative stress (thiobarbituric acid reactive substances and aconitase inactivation) by 82-116%. While broccoli sprouts increased total glutathione and activities of the phase 2 enzymes glutamate cysteine ligase and quinone reductase in liver, they did not affect these in ischemic-reperfused heart. While the mechanism is not clear, the results show that a relatively short dietary treatment with broccoli sprouts can strongly protect the heart against oxidative stress and cell death caused by ischemia-reperfusion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aconitate Hydratase / antagonists & inhibitors
  • Animals
  • Antioxidants / metabolism
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use*
  • Apoptosis / drug effects*
  • Brassica*
  • Caspase 3 / metabolism
  • Glutamate-Cysteine Ligase / metabolism
  • Glutathione / metabolism
  • Liver / metabolism
  • Male
  • Metabolic Detoxication, Phase II
  • Myocardial Ischemia / diet therapy*
  • Myocardial Ischemia / metabolism
  • Myocardial Reperfusion Injury / metabolism
  • Myocardial Reperfusion Injury / prevention & control*
  • NAD(P)H Dehydrogenase (Quinone) / metabolism
  • Necrosis / prevention & control
  • Oxidative Stress / drug effects*
  • Phytotherapy
  • Plant Preparations / pharmacology
  • Plant Preparations / therapeutic use*
  • Plant Shoots
  • Rats
  • Rats, Wistar
  • Thiobarbituric Acid Reactive Substances


  • Antioxidants
  • Plant Preparations
  • Thiobarbituric Acid Reactive Substances
  • NAD(P)H Dehydrogenase (Quinone)
  • Caspase 3
  • Aconitate Hydratase
  • Glutamate-Cysteine Ligase
  • Glutathione