[Genotype analysis of Giardia duodenalis isolates obtained from humans in west-central Poland]

Wiad Parazytol. 2010;56(2):171-7.
[Article in Polish]

Abstract

Giardia duodenalis (syn. G. intestinalis, G. lamblia) is a cosmopolitan flagellate organism belonging to the most common intestinal protozoan parasites of humans and animals. Great genetic heterogeneity has been found within G. duodenalis, where only genotypes representing assemblages A and B have zoonotic potential. Fecal samples (447 specimens) obtained from 232 humans in West-central region of Poland were examined by microscopy and PCR. The total prevalence of Giardia in humans was 1.3%. DNA was extracted from three positive fecal samples and PCR products were obtained after amplification using the beta-giardin primers G7 and G759. Sequence and phylogenetic analyses showed that G. duodenalis isolates from humans belonged to A and B genotypes. Moreover, three subgenotypes, including a cosmopolitan subgenotype A2 and two new subgenotypes A and B were detected.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Duodenum / parasitology*
  • Feces / parasitology*
  • Genotype
  • Giardia lamblia / classification*
  • Giardia lamblia / genetics*
  • Humans
  • Poland
  • Species Specificity