Elevation of oxidized DJ-1 in the brain and erythrocytes of Parkinson disease model animals

Neurosci Lett. 2010 Oct 15;483(3):201-5. doi: 10.1016/j.neulet.2010.08.007. Epub 2010 Aug 11.


DJ-1, the causative gene of a familial form of Parkinson's disease (PD), has been reported undergo oxidation preferentially at the 106th cysteine residue (Cys-106) under oxidative stress. Recently, it has been found that the levels of oxidized DJ-1 in erythrocytes of unmedicated PD patients are markedly higher than those in medicated PD patients and healthy subjects. In the present study, we examined the changes in oxidized DJ-1 levels in the brain and erythrocytes of PD animal models using specific antibodies against Cys-106-oxidized DJ-1. Treatment with PD model compounds such as 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 6-hydroxydopamine significantly elevated the levels of oxidized DJ-1 in erythrocytes. Immunohistochemical analysis also revealed that the number of oxidized DJ-1 antibody-positive cells in the substantia nigra of MPTP-treated mouse increased in a dose-dependent manner. These results suggest that the oxidative modification of DJ-1 in the brain and erythrocytes is involved in the pathogenesis of PD in animal models.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / pharmacology
  • Animals
  • Brain / drug effects
  • Brain / metabolism*
  • Catecholamines / metabolism
  • Chromatography, High Pressure Liquid / methods
  • Disease Models, Animal
  • Drug Administration Schedule
  • Enzyme-Linked Immunosorbent Assay / methods
  • Erythrocytes / drug effects
  • Erythrocytes / metabolism*
  • Mice
  • Oncogene Proteins / metabolism*
  • Oxidopamine / toxicity
  • Parkinson Disease / etiology
  • Parkinson Disease / pathology*
  • Peroxiredoxins
  • Protein Deglycase DJ-1
  • Rats
  • Time Factors


  • Catecholamines
  • Oncogene Proteins
  • Oxidopamine
  • 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
  • Peroxiredoxins
  • PARK7 protein, mouse
  • Protein Deglycase DJ-1