Disruption of quaternary structure in Escherichia coli dihydrodipicolinate synthase (DHDPS) generates a functional monomer that is no longer inhibited by lysine

Arch Biochem Biophys. 2010 Nov 15;503(2):202-6. doi: 10.1016/j.abb.2010.08.009. Epub 2010 Aug 13.


Escherichia coli dihydrodipicolinate synthase (DHDPS, E.C., a natively homotetrameric enzyme was converted to a monomeric species through the introduction of destabilising interactions at two different subunit interfaces allowing exploration of the roles of the quaternary structure in affecting catalytic competency. The double mutant DHDPS-L197D/Y107W displays gel filtration characteristics consistent with a single non-interacting monomeric species, which was confirmed by sedimentary velocity experiments. This monomer was shown to be catalytically active, but with reduced catalytic efficiency (k(cat)=9.8±0.5s(-1)), displaying 8% of the specific activity of the wild-type enzyme. The Michaelis constants for the substrates pyruvate and for (S)-aspartate semialdehyde increased by an order of magnitude, indicating that quaternary structure plays a significant role in substrate specificity. This monomeric species exhibited an enhanced propensity for aggregation and inactivation, indicating that whilst the oligomerization is not an intrinsic criterion for catalysis, higher oligomeric forms may benefit from both increased catalytic efficiency and diminished aggregation propensity. Furthermore, allosteric inhibition by (S)-lysine was abolished for DHDPS-L197D/Y107W, confirming the importance of the dimeric unit as the minimal functional assembly for efficient (S)-lysine binding.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Binding Sites
  • Catalysis
  • Enzyme Stability
  • Escherichia coli Proteins / chemistry*
  • Hydro-Lyases / chemistry*
  • Kinetics
  • Lysine / metabolism
  • Models, Molecular
  • Point Mutation
  • Protein Structure, Quaternary / physiology
  • Substrate Specificity


  • Escherichia coli Proteins
  • Hydro-Lyases
  • 4-hydroxy-tetrahydrodipicolinate synthase
  • Lysine