Development of a microwave-accelerated metal-enhanced fluorescence 40 second, <100 cfu/ml point of care assay for the detection of Chlamydia trachomatis

IEEE Trans Biomed Eng. 2011 Mar;58(3):781-4. doi: 10.1109/TBME.2010.2066275. Epub 2010 Aug 12.


An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with "bow-tie" structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new "release and detect" platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chlamydia trachomatis / isolation & purification*
  • DNA, Bacterial / chemistry
  • Microscopy, Electron, Transmission
  • Microwaves*
  • Molecular Diagnostic Techniques / methods*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Point-of-Care Systems*
  • Spectrometry, Fluorescence / methods*


  • DNA, Bacterial