Abstract
Cyclothiazomycin is a novel renin inhibitor produced by Streptomyces sp. NR0516. It was isolated from fermentation broth by extraction with butyl alcohol, QAE-Toyopearl column chromatography and preparative HPLC. Cyclothiazomycin, which was determined to be a unique polythiazole-containing bicyclic peptide, exhibited inhibitory activity against human plasma renin with IC50 being 1.7 microM.
MeSH terms
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Angiotensin I / analysis
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Animals
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Chromatography, High Pressure Liquid
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Fermentation
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Humans
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Peptides, Cyclic / biosynthesis
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Peptides, Cyclic / chemistry
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Peptides, Cyclic / isolation & purification
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Peptides, Cyclic / pharmacology*
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Radioimmunoassay
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Renin / antagonists & inhibitors*
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Soil Microbiology
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Spectrophotometry, Infrared
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Spectrophotometry, Ultraviolet
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Streptomyces / classification
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Streptomyces / metabolism*
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Thiazoles / chemistry
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Thiazoles / isolation & purification
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Thiazoles / pharmacology*
Substances
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Peptides, Cyclic
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Thiazoles
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cyclothiazomycin
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Angiotensin I
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Renin