The use of fibrous, supramolecular membranes and human tubular cells for renal epithelial tissue engineering: towards a suitable membrane for a bioartificial kidney

Macromol Biosci. 2010 Nov 10;10(11):1345-54. doi: 10.1002/mabi.201000146.

Abstract

A bioartificial kidney, which is composed of a membrane cartridge with renal epithelial cells, can substitute important kidney functions in patients with renal failure. A particular challenge is the maintenance of monolayer integrity and specialized renal epithelial cell functions ex vivo. We hypothesized that this can be improved by electro-spun, supramolecular polymer membranes which show clear benefits in ease of processability. We found that after 7 d, in comparison to conventional microporous membranes, renal tubular cells cultured on top of our fibrous supramolecular membranes formed polarized monolayers, which is prerequisite for a well-functioning bioartificial kidney. In future, these supramolecular membranes allow for incorporation of peptides that may increase cell function even further.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Antigens, Neoplasm / metabolism
  • Aquaporin 1 / metabolism
  • Bioartificial Organs*
  • CD13 Antigens / metabolism
  • Cell Adhesion Molecules / metabolism
  • Cell Survival
  • Cells, Cultured
  • Epithelial Cell Adhesion Molecule
  • Epithelial Cells / cytology*
  • Epithelial Cells / metabolism
  • Epithelial Cells / ultrastructure
  • Extracellular Matrix Proteins / genetics
  • Gene Expression / genetics
  • Glucose Transporter Type 1 / metabolism
  • Humans
  • Kidney Tubules / cytology*
  • Kidney*
  • Membrane Proteins / metabolism
  • Membrane Transport Proteins / genetics
  • Membranes, Artificial*
  • Microscopy, Electron, Transmission
  • Mitochondria / metabolism
  • Nanofibers / chemistry
  • Perfusion
  • Phosphoproteins / metabolism
  • Polyesters / chemistry
  • Polymers / chemical synthesis
  • Polymers / chemistry
  • Pyrimidinones / chemistry
  • Sodium-Potassium-Exchanging ATPase / metabolism
  • Tight Junctions / metabolism
  • Tight Junctions / ultrastructure
  • Tissue Engineering / methods*
  • Tissue Scaffolds / chemistry
  • Zonula Occludens-1 Protein
  • gamma-Glutamyltransferase / metabolism

Substances

  • 2-ureido-4-pyrimidone
  • AQP1 protein, human
  • Antigens, Neoplasm
  • Cell Adhesion Molecules
  • EPCAM protein, human
  • Epithelial Cell Adhesion Molecule
  • Extracellular Matrix Proteins
  • Glucose Transporter Type 1
  • Membrane Proteins
  • Membrane Transport Proteins
  • Membranes, Artificial
  • Phosphoproteins
  • Polyesters
  • Polymers
  • Pyrimidinones
  • SLC2A1 protein, human
  • TJP1 protein, human
  • Zonula Occludens-1 Protein
  • Aquaporin 1
  • polycaprolactone
  • gamma-Glutamyltransferase
  • gamma-glutamyltransferase, human
  • Alkaline Phosphatase
  • CD13 Antigens
  • Sodium-Potassium-Exchanging ATPase