The association of genomic variation of Epstein-Barr virus BamHI F fragment with the proliferation of nasopharyngeal carcinoma

APMIS. 2010 Sep 1;118(9):657-64. doi: 10.1111/j.1600-0463.2010.02642.x.


To investigate the f variant of Epstein-Barr virus (EBV) in nasopharyngeal carcinogenesis, we detected the f variant in primary nasopharyngeal carcinoma (NPC), metastatic carcinoma of the lymph node (LN), and chronic inflammation of the nasopharynx from the Guangdong region. Meanwhile, we analyzed the relationship between the f variant of EBV and LMP1, Fascin, pStat3, p53, Bcl-2, and Ki-67 expression in NPC. The results showed that the f variant of EBV was found in 11 cases of primary NPCs with LN metastasis, 12 LN metastases, and 18 primary NPCs without LN metastasis. However, only one demonstrated the F/f variant in 50 cases of chronic inflammation of the nasopharynx. The expression rate of LMP1, Fascin, pStat3, p53, Bcl-2, and Ki-67 in NPC with the f or F/f variant was higher than that with the F prototype. Furthermore, there was a significantly positive correlation between the f variant of EBV and Ki-67 expression (p < 0.05). Our study suggests that the f variant of EBV may be closely related to nasopharyngeal carcinogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Carrier Proteins / genetics
  • Deoxyribonuclease BamHI / genetics*
  • Epstein-Barr Virus Infections / genetics*
  • Female
  • Genetic Variation*
  • Herpesvirus 4, Human / genetics*
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Ki-67 Antigen / genetics
  • Male
  • Microfilament Proteins / genetics
  • Middle Aged
  • Nasopharyngeal Neoplasms / genetics*
  • Nasopharyngeal Neoplasms / pathology
  • Nasopharyngeal Neoplasms / virology
  • Neoplasm Metastasis / genetics
  • Neoplasm Metastasis / pathology
  • Peptide Fragments / genetics
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • TNF Receptor-Associated Factor 2 / genetics
  • Viral Proteins / genetics


  • Carrier Proteins
  • Ki-67 Antigen
  • Microfilament Proteins
  • Peptide Fragments
  • TNF Receptor-Associated Factor 2
  • Viral Proteins
  • fascin
  • Deoxyribonuclease BamHI