Pancreatic islet culture and preservation strategies: advances, challenges, and future outlook

Cell Transplant. 2010;19(12):1523-35. doi: 10.3727/096368910X515872. Epub 2010 Aug 17.

Abstract

Postisolation islet survival is a critical step for achieving successful and efficient islet transplantation. This involves the optimization of islet culture in order to prolong survival and functionality in vitro. Many studies have focused on different strategies to culture pancreatic islets in vitro through manipulation of culture media, surface modified substrates, and the use of various techniques such as encapsulation, embedding, scaffold, and bioreactor culture strategies. This review aims to present and discuss the different methodologies employed to optimize pancreatic islet culture in vitro as well as address their respective advantages and drawbacks.

Publication types

  • Review

MeSH terms

  • Animals
  • Bioreactors
  • Culture Media
  • Culture Techniques / instrumentation*
  • Culture Techniques / methods*
  • Drug Compounding
  • Humans
  • Islets of Langerhans / cytology*
  • Islets of Langerhans / physiology
  • Islets of Langerhans Transplantation / methods*
  • Preservation, Biological / methods*
  • Tissue Scaffolds

Substances

  • Culture Media