Novel characterization of monocyte-derived cell populations in the meninges and choroid plexus and their rates of replenishment in bone marrow chimeric mice

J Neuropathol Exp Neurol. 2010 Sep;69(9):896-909. doi: 10.1097/NEN.0b013e3181edbc1a.

Abstract

The mouse dura mater, pia mater, and choroid plexus contain resident macrophages and dendritic cells (DCs). These cells participate in immune surveillance, phagocytosis of cellular debris, uptake of antigens from the surrounding cerebrospinal fluid and immune regulation in many pathologic processes. We used Cx3cr1 knock-in, CD11c-eYFP transgenic and bone marrow chimeric mice to characterize the phenotype, density and replenishment rate of monocyte-derived cells in the meninges and choroid plexus and to assess the role of the chemokine receptor CX3CR1 on their number and tissue distribution. Iba-1 major histocompatibility complex (MHC) Class II CD169 CD68 macrophages and CD11c putative DCs were identified in meningeal and choroid plexus whole mounts. Comparison of homozygous and heterozygous Cx3cr1 mice did not reveal CX3CR1-dependancy on density, distribution or phenotype of monocyte-derived cells. In turnover studies, wild type lethally irradiated mice were reconstituted with Cx3cr1/-positive bone marrow and were analyzed at 3 days, 1, 2, 4 and 8 weeks after transplantation. There was a rapid replenishment of CX3CR1-positive cells in the dura mater (at 4 weeks) and the choroid plexus was fully reconstituted by 8 weeks. These data provide the foundation for future studies on the role of resident macrophages and DCs in conditions such as meningitis, autoimmune inflammatory disease and in therapies involving irradiation and hematopoietic or stem cell transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / genetics
  • Antigens, CD / metabolism
  • Antigens, Differentiation, Myelomonocytic / genetics
  • Antigens, Differentiation, Myelomonocytic / metabolism
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / physiology*
  • CD11c Antigen / genetics
  • CD11c Antigen / metabolism
  • CX3C Chemokine Receptor 1
  • Chimera*
  • Choroid Plexus / cytology*
  • Choroid Plexus / physiology
  • Dendritic Cells / metabolism
  • Gene Knock-In Techniques
  • Histocompatibility Antigens Class II / genetics
  • Histocompatibility Antigens Class II / metabolism
  • Humans
  • Immunophenotyping
  • Meninges / cytology*
  • Meninges / physiology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Transgenic
  • Monocytes / cytology
  • Monocytes / metabolism*
  • Receptors, Chemokine / genetics
  • Receptors, Chemokine / metabolism

Substances

  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD11c Antigen
  • CD68 antigen, human
  • CX3C Chemokine Receptor 1
  • Cx3cr1 protein, mouse
  • Histocompatibility Antigens Class II
  • Receptors, Chemokine