Functional proteomics to dissect tyrosine kinase signalling pathways in cancer

Nat Rev Cancer. 2010 Sep;10(9):618-29. doi: 10.1038/nrc2900. Epub 2010 Aug 19.

Abstract

Advances in the generation and interpretation of proteomics data have spurred a transition from focusing on protein identification to functional analysis. Here we review recent proteomics results that have elucidated new aspects of the roles and regulation of signal transduction pathways in cancer using the epidermal growth factor receptor (EGFR), ERK and breakpoint cluster region (BCR)-ABL1 networks as examples. The emerging theme is to understand cancer signalling as networks of multiprotein machines which process information in a highly dynamic environment that is shaped by changing protein interactions and post-translational modifications (PTMs). Cancerous genetic mutations derange these protein networks in complex ways that are tractable by proteomics.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Humans
  • Neoplasms / genetics
  • Neoplasms / metabolism*
  • Protein Binding
  • Protein Processing, Post-Translational
  • Protein-Tyrosine Kinases / chemistry*
  • Protein-Tyrosine Kinases / metabolism*
  • Proteomics*
  • Signal Transduction*

Substances

  • Protein-Tyrosine Kinases