Intragenic sequences affect the expression of the gene encoding glial fibrillary acidic protein

J Neurochem. 1991 Aug;57(2):675-84. doi: 10.1111/j.1471-4159.1991.tb03799.x.

Abstract

We show that the expression of the gene encoding glial fibrillary acidic protein (GFAP) gene is affected by at least three cis-acting elements. A positive regulatory element that is located between nucleotides -1,631 and -1,479 can confer cell type-specific expression on a heterologous gene. A second regulatory element is located between nucleotides -97 and -80. The third is a negative regulatory element that is located within the first intron of the gene. Deletion of this element activates GFAP expression in HeLa cells, and affects promoter function in glioma cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Astrocytoma
  • Cell Line
  • Chromosome Deletion
  • Gene Expression
  • Genes*
  • Genes, Regulator*
  • Glial Fibrillary Acidic Protein / genetics*
  • Glioma
  • HeLa Cells / physiology
  • Humans
  • Introns*
  • Plasmids

Substances

  • Glial Fibrillary Acidic Protein