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, 24 (1), 110-3

Role of Reactive Oxygen Species During Hypertension in Response to Chronic Antiangiogenic Factor (sFlt-1) Excess in Pregnant Rats

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Role of Reactive Oxygen Species During Hypertension in Response to Chronic Antiangiogenic Factor (sFlt-1) Excess in Pregnant Rats

Kiran B Tam Tam et al. Am J Hypertens.

Abstract

Background: Preeclampsia is associated with increased levels of reactive oxygen species (ROS) and the antiangiogenic factor, soluble fms-like tyrosine kinase-1 (sFlt-1). Moreover, recent studies have indicated that chronic sFlt-1 excess causes hypertension in pregnant animals. The purpose of this study was to evaluate the role of ROS in mediating sFlt-1-induced hypertension in the pregnant rat.

Methods: Mean arterial pressure (MAP), and plasma sFlt-1 and tissue ROS levels were measured in the following groups: (i) pregnant controls; (ii) sFlt-1-treated pregnant rats; (iii) Tempol-treated pregnant rats; (iv) sFlt-1- and Tempol-treated pregnant rats.

Results: MAP increased from 104 ± 2 mm Hg in pregnant control rats to 118 ± 3 mm Hg (P = 0.002) in sFlt-1-infused rats. Basal and nicotinamide adenine dinucleotide phosphate (NADPH)-stimulated levels of tissue ROS were increased in response to excess sFlt-1 during pregnancy. Pretreatment with Tempol attenuated oxidative stress and hypertension in response to sFlt-1.

Conclusions: ROS play an important role in mediating hypertension in response to chronic sFlt-1 excess during pregnancy.

Conflict of interest statement

Conflict of interest statement: All authors do not have any conflict of interest to declare.

Figures

Figure 1
Figure 1
Effect of chronic infusion of soluble fms-like tyrosine kinase (sFlt-1) on plasma sFlt-1 levels and meanarterial pressure (MAP) in pregnant rats in the presence and absence of Tempol treatment. Data are expressed as mean ± SEM, * indicates P< 0.05.
Figure 2
Figure 2
Effect of chronic infusion of soluble fms-like tyrosine kinase (sFlt-1) on basal and NADPH stimulated vascular superoxide levels in pregnant rats in the presence and absence of Tempol treatment. Data are expressed as mean ± SEM, * indicates P < 0.05.

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