The impact of plasma rich in growth factors on clinical and biological factors involved in healing processes after third molar extraction

J Biomed Mater Res A. 2010 Dec 1;95(3):741-6. doi: 10.1002/jbm.a.32882.


Extraction of an impacted mandibular third molar is a common surgical procedure, although it still leads to several postoperative symptoms and complications. The study assessed the efficacy of autologous plasma rich in growth factors (PRGF) in the healing process by checking the difference of tissue cytokines and other healing factors produced by the mucosa after extraction between sites treated with PRGF and control sites and, at the same time, by evaluating the clinical efficacy of PRGF in terms of reduced pain and facial swelling. This study was a split-mouth study, in which the patient becomes his/her own control, to eliminate any individual response differences toward PRGF treatment. The parameters regarding inflammation and subsequent wound healing were all significantly higher at PRGF sites than at control sites. The increase at PRGF sites of the two proinflammatory cytokines evaluated, interleukin (IL)-1β and IL-6, was accompanied by the increase of two anti-inflammatory cytokines, IL-10 and transforming growth factor-β. Furthermore, IL-1β and IL-6 induce fibroblast and keratinocyte proliferation, important events in wound healing. Postoperative pain and the swelling, measured at all experimental times, were reduced in the presence of PRGF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Biological Factors* / blood
  • Biological Factors* / pharmacology
  • Humans
  • Inflammation / immunology
  • Intercellular Signaling Peptides and Proteins* / blood
  • Intercellular Signaling Peptides and Proteins* / pharmacology
  • Interleukin-10 / immunology
  • Interleukin-1beta / immunology
  • Interleukin-6 / immunology
  • Molar, Third / surgery*
  • Pain, Postoperative
  • Plasma / chemistry*
  • Random Allocation
  • Tooth Extraction*
  • Transforming Growth Factor beta / immunology
  • Wound Healing / drug effects*
  • Young Adult


  • Biological Factors
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-1beta
  • Interleukin-6
  • Transforming Growth Factor beta
  • Interleukin-10