Development of chitosan-collagen hydrogel incorporated with lysostaphin (CCHL) burn dressing with anti-methicillin-resistant Staphylococcus aureus and promotion wound healing properties

Drug Deliv. 2011 Apr;18(3):173-80. doi: 10.3109/10717544.2010.509363. Epub 2010 Aug 21.

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) have become increasingly prevalent as nosocomial pathogens, especially in burn patients, which is the leading cause of their death. A drug delivery system of chitosan-collagen hydrogel incorporated with lysostaphin (CCHL) based on the lysostaphin gauze was developed for MRSA infected burn wounds. CCHL scaffold consisted of numerous interconnected sphericles and tubular bodies with an average diameter of 100-200 µm, 20-60-fold swelling, high water retention capacity, and cell proliferation properties. The minimal inhibitory concentration of CCHL was 0.053 U/mL. By the second week after its application on MRSA infected third-degree burn wounds, no bacteria could be detected and the burn wounds had started healing. Therefore, CCHL should be studied further as a promising candidate of burn treatment dressing against MRSA infections for clinics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / administration & dosage
  • Anti-Bacterial Agents / pharmacology*
  • Bandages
  • Burns / drug therapy*
  • Burns / microbiology
  • Burns / pathology
  • Cell Proliferation
  • Cells, Cultured
  • Chitosan / chemistry
  • Collagen / chemistry
  • Drug Delivery Systems
  • Humans
  • Hydrogels
  • Lysostaphin / administration & dosage
  • Lysostaphin / pharmacology*
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification
  • Microbial Sensitivity Tests
  • Rabbits
  • Staphylococcal Infections / drug therapy*
  • Staphylococcal Infections / microbiology
  • Wound Healing / drug effects
  • Wound Infection / drug therapy
  • Wound Infection / microbiology

Substances

  • Anti-Bacterial Agents
  • Hydrogels
  • Collagen
  • Chitosan
  • Lysostaphin