Low Access Resistance Perforated Patch Recordings Using Amphotericin B

J Neurosci Methods. 1991 Mar;37(1):15-26. doi: 10.1016/0165-0270(91)90017-t.

Abstract

We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 M omega were achieved and with blunt electrode tips, access resistances of 3-4 M omega were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amphotericin B / chemistry
  • Amphotericin B / pharmacology*
  • Animals
  • Anura
  • Cells, Cultured
  • Electrodes
  • Electrophysiology / instrumentation*
  • Epithelial Cells
  • Epithelium / physiology
  • Fishes
  • Guinea Pigs
  • Humans
  • Lens, Crystalline / physiology
  • Mice
  • Nystatin / pharmacology
  • Rabbits
  • Rats
  • Solutions
  • Tumor Cells, Cultured / drug effects

Substances

  • Solutions
  • Nystatin
  • Amphotericin B