Rapid selection of transgenic C. elegans using antibiotic resistance

Nat Methods. 2010 Sep;7(9):725-7. doi: 10.1038/nmeth.1495. Epub 2010 Aug 22.


Caenorhabditis elegans is an important model organism in biology, but until now no antibiotic selection markers have been successfully demonstrated for this species. We have developed a selection system using puromycin that allows the rapid and easy isolation of large populations of transgenic worms. This approach is sufficiently powerful to select single-copy transgenes, does not require any particular genetic background and also works in C. briggsae.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Caenorhabditis elegans / drug effects*
  • Caenorhabditis elegans / genetics*
  • Drug Resistance / drug effects*
  • Drug Resistance / genetics*
  • Genetic Markers
  • Genetic Vectors
  • Puromycin / pharmacology
  • Selection, Genetic / drug effects*
  • Time Factors
  • Transgenes / genetics*


  • Genetic Markers
  • Puromycin