Rapid selection of transgenic C. elegans using antibiotic resistance

Nat Methods. 2010 Sep;7(9):725-7. doi: 10.1038/nmeth.1495. Epub 2010 Aug 22.

Abstract

Caenorhabditis elegans is an important model organism in biology, but until now no antibiotic selection markers have been successfully demonstrated for this species. We have developed a selection system using puromycin that allows the rapid and easy isolation of large populations of transgenic worms. This approach is sufficiently powerful to select single-copy transgenes, does not require any particular genetic background and also works in C. briggsae.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Caenorhabditis elegans / drug effects*
  • Caenorhabditis elegans / genetics*
  • Drug Resistance / drug effects*
  • Drug Resistance / genetics*
  • Genetic Markers
  • Genetic Vectors
  • Puromycin / pharmacology
  • Selection, Genetic / drug effects*
  • Time Factors
  • Transgenes / genetics*

Substances

  • Genetic Markers
  • Puromycin