An Antibiotic Selection Marker for Nematode Transgenesis

Nat Methods. 2010 Sep;7(9):721-3. doi: 10.1038/nmeth.1494. Epub 2010 Aug 22.

Abstract

We have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (NeoR) and shown that it could confer resistance to G-418 on both wild-type Caenorhabditis elegans and C. briggsae. This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates. We also show that this marker can be used for Mos1-mediated single-copy insertion in wild-type genetic backgrounds (MosSCI-biotic).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Caenorhabditis / classification
  • Caenorhabditis / drug effects*
  • Caenorhabditis / genetics*
  • Drug Resistance / drug effects
  • Drug Resistance / genetics*
  • Genetic Markers / genetics
  • Genetic Vectors / genetics
  • Gentamicins / pharmacology*
  • Neomycin / pharmacology
  • Selection, Genetic / drug effects*
  • Selection, Genetic / genetics*
  • Transgenes / genetics*

Substances

  • Genetic Markers
  • Gentamicins
  • antibiotic G 418
  • Neomycin