Canine adipose-derived-mesenchymal stem cells do not lose stem features after a long-term cryopreservation

Res Vet Sci. 2011 Aug;91(1):18-24. doi: 10.1016/j.rvsc.2010.07.024. Epub 2010 Aug 21.


Adult stem cells are nowadays used for treating several pathologies. A putative stem cell population was found in the adipose tissue of mammals and canine adipose tissue-derived-mesenchymal stem cells (cA-MSC) have been shown to possess the capacity to differentiate into several lineages. The main goal of our research was to fully characterize cA-MSC and examine the effects of cryopreservation on their stemness features. Each sample of cA-MSC was analyzed immediately and then again after being frozen in liquid nitrogen for one year. After the cryopreservation period cells conserved their fibroblast-like morphology, alkaline phosphatase positivity and CD expression but showed a lower proliferation ratio and a lower telomerase activity in comparison with fresh cells. Finally, the cryopreservation protocol did not change the cA-MSC adipogenic, osteogenic and myogenic differentiative potential. Our data demonstrate that stored cA-MSC might represent a promising type of progenitor cell for autologous cellular-based therapies in veterinary medicine.

MeSH terms

  • Adipose Tissue / cytology*
  • Alkaline Phosphatase / metabolism
  • Animals
  • Antigens, Surface / metabolism
  • Cell Culture Techniques / veterinary
  • Cell Differentiation
  • Cryopreservation / veterinary*
  • Dogs
  • Female
  • Flow Cytometry / veterinary
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Mesenchymal Stem Cells / physiology*
  • Polymerase Chain Reaction / veterinary
  • Sequence Analysis, RNA / veterinary
  • Telomerase / metabolism


  • Antigens, Surface
  • Telomerase
  • Alkaline Phosphatase