MicroRNA-10b induced by Epstein-Barr virus-encoded latent membrane protein-1 promotes the metastasis of human nasopharyngeal carcinoma cells

Cancer Lett. 2010 Dec 18;299(1):29-36. doi: 10.1016/j.canlet.2010.07.021. Epub 2010 Aug 21.


MicroRNA-10b (miR-10b) has been reported to facilitate the metastasis of breast cancer. However, little is known about the role of miR-10b in the metastasis of nasopharyngeal carcinoma (NPC). Here, we show that high levels of miR-10b expression in Epstein-Barr virus (EBV)-positive latent membrane protein-1 (LMP1)-expressing NPC cells, and its expression is down-regulated by silencing LMP1 or Twist. Induction of miR-10b over-expression in LMP1-silent C666-1 cells promoted significant wound healing and transmembrane invasiveness in vitro. More importantly, miR-10b over-expression promoted the metastasis of NPC and accelerated the death of tumor-bearing nude mice. These findings strongly suggest that miR-10b positively regulates the metastasis of NPC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Movement
  • Herpesvirus 4, Human / physiology*
  • Humans
  • Male
  • Mice
  • Mice, Inbred BALB C
  • MicroRNAs / analysis
  • MicroRNAs / physiology*
  • Nasopharyngeal Neoplasms / pathology*
  • Nasopharyngeal Neoplasms / virology
  • Neoplasm Invasiveness
  • Neoplasm Metastasis
  • Twist-Related Protein 1 / physiology
  • Viral Matrix Proteins / analysis
  • Viral Matrix Proteins / physiology*


  • EBV-associated membrane antigen, Epstein-Barr virus
  • MIRN10 microRNA, human
  • MicroRNAs
  • Twist-Related Protein 1
  • Viral Matrix Proteins