Worldwide Diversity of Klebsiella Pneumoniae That Produce Beta-Lactamase blaKPC-2 Gene

Emerg Infect Dis. 2010 Sep;16(9):1349-56. doi: 10.3201/eid1609.091389.

Abstract

Klebsiella pneumoniaeisolates that produce carbapenemases (KPCs) are rapidly disseminating worldwide. To determine their genetic background, we investigated 16 blaKPC-2-harboring K. pneumoniae isolates from 5 countries. The isolates were multidrug resistant, possessed the blaKPC-2 gene, and differed by additional Beta-lactamase content. They harbored a naturally chromosome-encoded bla gene (blaSHV-1 [12.5%], blaSHV-11 [68.7%], or blaOKP-AVB [18.8%]) and several acquired and plasmid-encoded genes (blaTEM-1 [81.3%], blaCTX-M-2 [31.3%], blaCTX-M-12 [12.5%], blaCTX-M-15 [18.7%], and blaOXA-9 [37.5%]). The blaKPC-2 gene was always associated with 1 of the Tn4401 isoforms (a, b, or c). Tn4401 was inserted on different-sized plasmids that belonged to different incompatibility groups. Several blaKPC-containing K. pneumoniae clones were found: 9 different pulsotypes with 1 major (sequence type 258) and 7 minor distinct allelic profiles. Different clones harboring different plasmids but having identical genetic structure, Tn4401, could be at the origin of the worldwide spread of this emerging resistance gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Sequence
  • Communicable Diseases, Emerging / epidemiology
  • Communicable Diseases, Emerging / microbiology*
  • DNA Primers / genetics
  • DNA Transposable Elements / genetics
  • DNA, Bacterial / genetics
  • Drug Resistance, Multiple, Bacterial / genetics
  • Genes, Bacterial
  • Genetic Variation
  • Global Health
  • Humans
  • Klebsiella Infections / epidemiology
  • Klebsiella Infections / microbiology*
  • Klebsiella pneumoniae / classification
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / enzymology*
  • Klebsiella pneumoniae / genetics*
  • Plasmids / genetics
  • Replication Origin
  • beta-Lactamases / biosynthesis*
  • beta-Lactamases / genetics*

Substances

  • DNA Primers
  • DNA Transposable Elements
  • DNA, Bacterial
  • beta-Lactamases
  • beta-lactamase KPC-2, Klebsiella pneumoniae