Lack of EP4 receptors on bone marrow-derived cells enhances inflammation in atherosclerotic lesions

Cardiovasc Res. 2011 Jan 1;89(1):234-43. doi: 10.1093/cvr/cvq262. Epub 2010 Aug 24.


Aim: prostaglandin E(2), by ligation of its receptor EP4, suppresses the production of inflammatory cytokines and chemokines in macrophages in vitro. Thus, activation of EP4 may constitute an endogenous anti-inflammatory pathway. This study investigated the role of EP4 in atherosclerosis in vivo, and particularly its impact on inflammation.

Methods and results: Ldlr(-/-) mice transplanted with EP4(+/+) or EP4(-/-) bone marrow consumed a high-fat diet for 5 or 10 weeks. Allogenic bone marrow transplantation promoted exacerbation of atherosclerosis irrespective of EP4 genotype, compatible with prior observations of exacerbated atherogenesis by allogenicity. EP4 deficiency had little effect on plaque size or morphology in early atherosclerosis, but at the later time point, mice deficient in EP4 displayed enhanced inflammation in their atherosclerotic plaques. Expression of monocyte chemoattractant protein-1 and interferon-γ inducible protein 10 increased, and there was a corresponding increase in macrophage and T-cell infiltration. These plaques also contained fewer smooth muscle cells. Despite these changes, mice deficient in EP4 in bone marrow-derived cells at an advanced stage had similar lesion size (in both aorta and aortic root) as mice with EP4.

Conclusion: this study shows that in advanced atherosclerosis, EP4 deficiency did not alter atherosclerotic lesion size, but yielded plaques with exacerbated inflammation and altered lesion composition.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Bone Marrow Cells / metabolism*
  • Bone Marrow Cells / pathology
  • Bone Marrow Transplantation
  • Chemokine CCL2 / genetics
  • Chemokine CXCL10 / genetics
  • DNA Primers / genetics
  • Female
  • Inflammation / metabolism
  • Inflammation / pathology
  • Male
  • Mice
  • Mice, 129 Strain
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Mice, Knockout
  • Models, Cardiovascular
  • Plaque, Atherosclerotic / genetics
  • Plaque, Atherosclerotic / metabolism*
  • Plaque, Atherosclerotic / pathology
  • Receptors, LDL / deficiency
  • Receptors, LDL / genetics
  • Receptors, Prostaglandin E, EP4 Subtype / deficiency*
  • Receptors, Prostaglandin E, EP4 Subtype / genetics
  • Receptors, Prostaglandin E, EP4 Subtype / metabolism


  • Ccl2 protein, mouse
  • Chemokine CCL2
  • Chemokine CXCL10
  • Cxcl10 protein, mouse
  • DNA Primers
  • Receptors, LDL
  • Receptors, Prostaglandin E, EP4 Subtype