Abstract
We tested the hypothesis that caveolin-3 (Cav-3) is essential for opioid-induced preconditioning in vivo. Cav-3 overexpressing mice, Cav-3 knockout mice, and controls were exposed to myocardial ischemia/reperfusion (I/R) in the presence of SNC-121 (SNC), a δ-selective opioid agonist, or naloxone, a nonselective opioid antagonist. Controls were protected from I/R injury by SNC. No protection was produced by SNC in Cav-3 knockout mice. Cav-3 overexpressing mice showed innate protection from I/R compared with controls that was abolished by naloxone. Our results show that opioid-induced preconditioning is dependent on Cav-3 expression and that endogenous protection in Cav-3 overexpressing mice is opioid dependent.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Analgesics, Opioid / administration & dosage*
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Animals
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Benzamides / administration & dosage*
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Blood Pressure / drug effects
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Caveolin 3 / deficiency
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Caveolin 3 / genetics
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Caveolin 3 / metabolism*
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Disease Models, Animal
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Drug Administration Schedule
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Heart Rate / drug effects
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, Transgenic
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Myocardial Infarction / genetics
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Myocardial Infarction / metabolism
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Myocardial Infarction / pathology
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Myocardial Infarction / prevention & control*
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Myocardial Reperfusion Injury / genetics
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Myocardial Reperfusion Injury / metabolism
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Myocardial Reperfusion Injury / pathology
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Myocardial Reperfusion Injury / prevention & control*
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Myocardium / metabolism*
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Myocardium / pathology
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Naloxone / administration & dosage
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Narcotic Antagonists / administration & dosage
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Piperazines / administration & dosage*
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Receptors, Opioid, delta / agonists*
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Receptors, Opioid, delta / antagonists & inhibitors
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Receptors, Opioid, delta / metabolism
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Time Factors
Substances
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Analgesics, Opioid
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Benzamides
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Cav3 protein, mouse
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Caveolin 3
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Narcotic Antagonists
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Piperazines
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Receptors, Opioid, delta
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SNC 121
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Naloxone