Interaction of PqqE and PqqD in the Pyrroloquinoline Quinone (PQQ) Biosynthetic Pathway Links PqqD to the Radical SAM Superfamily

Chem Commun (Camb). 2010 Oct 7;46(37):7031-3. doi: 10.1039/c0cc00968g. Epub 2010 Aug 25.

Abstract

pqqD is one of six genes required for PQQ production in Klebsiella pneumoniae. Herein, we demonstrate that PqqD interacts specifically with the radical SAM enzyme PqqE, causing a perturbation in the electronic environment around the [4Fe-4S](+) clusters. This interaction redirects the role for PqqD in PQQ biosynthesis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism
  • Free Radicals / chemistry
  • Free Radicals / metabolism
  • Klebsiella pneumoniae / enzymology
  • PQQ Cofactor / biosynthesis*
  • PQQ Cofactor / chemistry
  • S-Adenosylmethionine / chemistry*
  • S-Adenosylmethionine / metabolism

Substances

  • Bacterial Proteins
  • Free Radicals
  • PQQ Cofactor
  • S-Adenosylmethionine