Solution structure of the HRDC domain of human Bloom syndrome protein BLM

J Biochem. 2010 Oct;148(4):517-25. doi: 10.1093/jb/mvq097. Epub 2010 Aug 25.

Abstract

Bloom syndrome is a rare genetic disorder characterized by severe growth retardation and cancer predisposition. The disease is caused by a loss of function of the Bloom syndrome protein (BLM), a member of the RecQ family of DNA helicases. Here we report on the first 3D structure of a BLM fragment, a solution structure of the C-terminal helicase-and-ribonuclease D-C-terminal (HRDC) domain from human BLM. The structure reveals unique features of BLM HRDC that are distinct from the HRDC domain of Werner syndrome protein. In particular, BLM HRDC retains many acidic residues exposed to the solvent, which makes the domain surface extensively electronegative. Consistent with this, fluorescence polarization assays showed an inability of isolated BLM HRDC to interact with DNA substrates. Analyses employing ultracentrifugation, gel-filtration, CD spectroscopy and dynamic light scattering showed that the BLM HRDC domain exists as a stable monomer in solution. The results show that BLM HRDC is a compact, robust and acidic motif which may play a distinct role apart from DNA binding.

MeSH terms

  • Amino Acid Sequence
  • Bloom Syndrome / genetics
  • Exodeoxyribonucleases / genetics
  • Humans
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • Protein Structure, Tertiary*
  • RecQ Helicases / chemistry*
  • RecQ Helicases / genetics
  • Sequence Homology, Amino Acid
  • Werner Syndrome Helicase

Substances

  • Exodeoxyribonucleases
  • Bloom syndrome protein
  • RecQ Helicases
  • WRN protein, human
  • Werner Syndrome Helicase

Associated data

  • PDB/2RRD