Permissive environment for B-cell maturation in myositis muscle in the absence of B-cell follicles

Muscle Nerve. 2010 Oct;42(4):576-83. doi: 10.1002/mus.21739.


Myositis muscle contains antigen-matured B-cells and plasma cells. Myositis muscle biopsy specimens were examined for nodular collections of T-cells, B-cells, myeloid dendritic cells, plasma cells, and follicular dendritic cells. Immunoglobulin and B-cell-activating factor (BAFF) transcripts were quantitated. Laser-capture microdissection was used to isolate single plasma cells, and their immunoglobulin transcripts were sequenced. Dense inflammatory infiltrates contained histological elements of ectopic lymphoid tissue but not B-cell follicles. Immunoglobulin transcript sequence analysis demonstrated spatially distributed, clonally related B-cells and plasma cells, suggesting local maturation of B-cells into plasma cells in myositis muscle. Regions of dense cellular infiltrates in myositis muscle are sometimes areas of B-cell maturation into antibody-producing plasma cells. An atypical lymphoid histology, lacking concentrated collections of germinal-center-like B-cell follicles, is capable of antigen-stimulated clonal maturation of antibody-producing plasma cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigen Presentation
  • B-Cell Activating Factor / genetics
  • B-Cell Maturation Antigen / pharmacology
  • B-Lymphocytes* / drug effects
  • B-Lymphocytes* / metabolism
  • Cellular Senescence* / drug effects
  • Choristoma / pathology
  • Dendritic Cells / immunology
  • Dendritic Cells / pathology
  • Germinal Center
  • Humans
  • Immunoglobulins / genetics
  • In Vitro Techniques
  • Lymphoid Tissue
  • Microdissection / methods
  • Muscle, Skeletal / pathology*
  • Muscle, Skeletal / physiopathology*
  • Muscular Diseases / pathology
  • Myositis / physiopathology*
  • Plasma Cells / metabolism
  • Plasma Cells / pathology
  • RNA, Messenger / metabolism
  • T-Lymphocytes, Helper-Inducer / immunology
  • T-Lymphocytes, Helper-Inducer / pathology


  • B-Cell Activating Factor
  • B-Cell Maturation Antigen
  • Immunoglobulins
  • RNA, Messenger