Cellular and tumoural heterogeneity of EGFR gene amplification in human malignant gliomas

Acta Neurochir (Wien). 1990;107(3-4):82-7. doi: 10.1007/BF01405784.


It has been demonstrated that the epidermal growth factor receptor (EGFR) gene, the normal human counterpart of the viral erb-B oncogene is amplified and overexpressed in over 50% of human malignant gliomas (HMGs). In the present study, analysis of the immunohistological staining characteristics of 57 HMGs using an anti-EGFR monoclonal antibody (mab) showed positive staining in 65% of the tumours with large cellular and regional differences in staining pattern and intensity. Screening a smaller number of HMGs with molecular hybridization techniques revealed 10/21 glioblastomas (48%) amplified for the gene; of 11 glioblastomas studied by Northern blot hybridization, 7 tumours with gene amplification showed RNA overexpression, the remaining 4 without amplification did not. Regional differences in DNA levels were observed by Southern blot in 2 tumours: in one particular case, amplification and overexpression were found to be localized to one half of a single HMG, the other half showing neither EFGR gene amplification nor overexpression.

MeSH terms

  • Blotting, Northern
  • Blotting, Southern
  • Brain / pathology
  • Brain Neoplasms / genetics*
  • Brain Neoplasms / pathology
  • DNA, Neoplasm / genetics
  • ErbB Receptors
  • Gene Amplification / genetics*
  • Gene Expression Regulation, Neoplastic / physiology*
  • Glioma / genetics*
  • Glioma / pathology
  • Humans
  • Immunoenzyme Techniques
  • Proto-Oncogene Proteins / genetics
  • RNA, Neoplasm / genetics


  • DNA, Neoplasm
  • Proto-Oncogene Proteins
  • RNA, Neoplasm
  • ErbB Receptors