Suitability of 2-deoxyglucose for Measuring Initial Rates of Glucose Uptake in Isolated Adipocytes

Biochem Int. 1990 Nov;22(4):607-15.

Abstract

The suitability of [3H]-2-deoxyglucose from measuring initial rates of glucose uptake in isolated rat adipocytes was assessed using three approaches. Basal and insulin-stimulated rates of glucose uptake were directly compared in 2 sec and 5 min assays using [14C]-3-O-methylglucose, [3H]-2-deoxyglucose, and [3H]-D-glucose. Equilibrium kinetics of 2-deoxyglucose uptake were compared with those of 3-O-methylglucose through impairment of hexokinase activity by depleting cellular energy with 2,4-dinitrophenol. The equivalence of these glucose analogues in a dynamic system was assessed by measuring the lag time preceding insulin stimulation of glucose uptake, insulin activation rates, and the T 1/2 of insulin activation. Our results demonstrate that no fundamental difference exists in the initial transport of 3-O-methylglucose, 2-deoxyglucose, and D-glucose.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 2,4-Dinitrophenol
  • 3-O-Methylglucose
  • Adipose Tissue / cytology
  • Adipose Tissue / metabolism*
  • Animals
  • Biological Transport
  • Deoxyglucose / metabolism*
  • Dinitrophenols / pharmacology
  • Glucose / metabolism*
  • Hexokinase / metabolism
  • Insulin / pharmacology
  • Kinetics
  • Male
  • Methylglucosides / metabolism
  • Rats
  • Rats, Inbred Strains

Substances

  • Dinitrophenols
  • Insulin
  • Methylglucosides
  • 3-O-Methylglucose
  • Deoxyglucose
  • Hexokinase
  • Glucose
  • 2,4-Dinitrophenol