The type IV pilin, PilA, is required for full virulence of Francisella tularensis subspecies tularensis

BMC Microbiol. 2010 Aug 26;10:227. doi: 10.1186/1471-2180-10-227.

Abstract

Background: All four Francisella tularensis subspecies possess gene clusters with potential to express type IV pili (Tfp). These clusters include putative pilin genes, as well as pilB, pilC and pilQ, required for secretion and assembly of Tfp. A hallmark of Tfp is the ability to retract the pilus upon surface contact, a property mediated by the ATPase PilT. Interestingly, out of the two major human pathogenic subspecies only the highly virulent type A strains have a functional pilT gene.

Results: In a previous study, we were able to show that one pilin gene, pilA, was essential for virulence of a type B strain in a mouse infection model. In this work we have examined the role of several Tfp genes in the virulence of the pathogenic type A strain SCHU S4. pilA, pilC, pilQ, and pilT were mutated by in-frame deletion mutagenesis. Interestingly, when mice were infected with a mixture of each mutant strain and the wild-type strain, the pilA, pilC and pilQ mutants were out-competed, while the pilT mutant was equally competitive as the wild-type.

Conclusions: This suggests that expression and surface localisation of PilA contribute to virulence in the highly virulent type A strain, while PilT was dispensable for virulence in the mouse infection model.

MeSH terms

  • Animals
  • Female
  • Fimbriae Proteins / genetics
  • Fimbriae Proteins / metabolism*
  • Francisella tularensis / genetics
  • Francisella tularensis / metabolism*
  • Francisella tularensis / pathogenicity*
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Mutation
  • Random Allocation
  • Virulence
  • Virulence Factors / genetics
  • Virulence Factors / metabolism*

Substances

  • PilA protein, Francisella tularensis
  • Virulence Factors
  • Fimbriae Proteins