Increased intramuscular nerve branching and inhibition of programmed cell death of chick embryo motoneurons by immunoglobulins from patients with motoneuron disease

J Neuroimmunol. 2010 Dec 15;229(1-2):157-68. doi: 10.1016/j.jneuroim.2010.07.028.

Abstract

Massive programmed cell death (PCD) of developing chick embryo motoneurons (MNs) occurs in a well defined temporal and spatial sequence between embryonic day (E) 6 and E10. We have found that, when administered in ovo, either circulating immunoglobulins G (IgGs) or cerebrospinal fluid from patients with MN disease can rescue a significant number of chick embryo MNs from normally occurring PCD. An increase of branching of intramuscular nerves was also observed that may account for the rescuing effects of pathologic IgGs. Proteomic analysis and further analysis by ELISA indicated that these effects may be mediated by the interaction of circulating human immunoglobulins with proteins of the semaphorin family.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Apoptosis / drug effects*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Chick Embryo
  • Chlorocebus aethiops
  • Dose-Response Relationship, Drug
  • Electrophoresis, Gel, Two-Dimensional / methods
  • Enzyme-Linked Immunosorbent Assay / methods
  • Female
  • Ganglia, Spinal / cytology
  • Growth Cones / drug effects
  • Humans
  • Immunoglobulins / immunology
  • Immunoglobulins / pharmacology*
  • In Vitro Techniques
  • Male
  • Motor Neuron Disease / blood
  • Motor Neuron Disease / immunology*
  • Motor Neurons / cytology
  • Motor Neurons / drug effects*
  • Muscle, Skeletal / embryology
  • Muscle, Skeletal / innervation*
  • Neuromuscular Junction / drug effects*
  • Neuromuscular Junction / physiology
  • Proteomics / methods
  • Semaphorins / metabolism
  • Serum / chemistry
  • Serum / immunology
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • Statistics as Topic
  • Statistics, Nonparametric
  • Transfection / methods
  • Tubulin / metabolism

Substances

  • Immunoglobulins
  • Semaphorins
  • Tubulin