A HPLC method to evaluate the influence of photostabilizers on cosmetic formulations containing UV-filters and vitamins A and E

Lorena Rigo Gaspar; Patricia Maria Berardo Gonçalves Maia Campos

doi:10.1016/j.talanta.2010.07.025

A HPLC method to evaluate the influence of photostabilizers on cosmetic formulations containing UV-filters and vitamins A and E

Talanta. 2010 Sep 15;82(4):1490-4. doi: 10.1016/j.talanta.2010.07.025. Epub 2010 Jul 17.

Authors

Lorena Rigo Gaspar¹, Patricia Maria Berardo Gonçalves Maia Campos

Affiliation

¹ University of Sao Paulo, Faculty of Pharmaceutical Sciences of Ribeirao Preto, Sao Paulo, Brazil. lorena@fcfrp.usp.br

PMID: 20801361
DOI: 10.1016/j.talanta.2010.07.025

Abstract

This paper reports a simple and reliable HPLC method to evaluate the influence of two currently available photostabilizers on cosmetic formulations containing combined UV-filters and vitamins A and E. Vitamins and UV-filters, widely encountered in products of daily use have to be routinely evaluated since photoinstability can lead to reductions in their efficacy and safety. UV-irradiated formulation samples were submitted to a procedure that included a reliable, precise and specific HPLC method employing a C18 column and detection at 325 and 235 nm. Methanol, isopropanol and water were the mobile phases in gradient elution. The method precision was between 0.28 and 5.07. The photostabilizers studied [diethylhexyl 2,6-naphthalate (DEHN) and benzotriazolyl dodecyl p-cresol (BTDC)], influenced the stability of octyl methoxycinnamate (OMC) associated with vitamins A and E. BTDC was considered the best photostabilizer to vitamins and OMC when the UV-filters were combined with both vitamins A and E.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid / methods*
Cosmetics / chemistry*
Photochemistry*
Reproducibility of Results
Ultraviolet Rays*
Vitamin A / analysis*
Vitamin E / analysis*

Substances

Cosmetics
Vitamin A
Vitamin E