All oligosaccharide moieties of the μ chains in the pre-BCR are of the high-mannose type

Mol Immunol. Nov-Dec 2010;48(1-3):351-5. doi: 10.1016/j.molimm.2010.07.005.

Abstract

Although it is well established that pre-BCR signaling governs proliferation and differentiation during B cell development, the components of the pre-BCR that are important for signaling are a matter of controversy. It has been suggested that signaling by the μ heavy chains of the pre-BCR induces survival and differentiation of pre-B cells, while the λ5 part of the pre-BCR is essential for proliferation and clonal expansion. However, the mechanism by which pre-BCR μ chains initiate differentiation signals is not clear. Using two variants of a murine B-lymphocyte cell line that differ only in surface expression of either BCR or pre-BCR, we demonstrated that surface μ chains in the pre-BCR are of the high-mannose type only, while those in the BCR are of the complex type. It is hypothesized that mannose-specific lectin-like molecules on accessory cells or in solution may function as the non-antigen ligand that triggers the pre-BCR.

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Differentiation / immunology
  • Cell Separation
  • Flow Cytometry
  • Immunoglobulin mu-Chains / chemistry*
  • Immunoglobulin mu-Chains / immunology
  • Immunoprecipitation
  • Mannose / chemistry*
  • Mannose / immunology
  • Mannose-Binding Lectins / chemistry*
  • Mannose-Binding Lectins / immunology
  • Mice
  • Oligosaccharides
  • Pre-B Cell Receptors / chemistry*
  • Pre-B Cell Receptors / immunology
  • Precursor Cells, B-Lymphoid / chemistry
  • Precursor Cells, B-Lymphoid / cytology
  • Precursor Cells, B-Lymphoid / immunology

Substances

  • Immunoglobulin mu-Chains
  • Mannose-Binding Lectins
  • Oligosaccharides
  • Pre-B Cell Receptors
  • Mannose