Rapidly denoising pyrosequencing amplicon reads by exploiting rank-abundance distributions

Nat Methods. 2010 Sep;7(9):668-9. doi: 10.1038/nmeth0910-668b.

Abstract

We developed a fast method for denoising pyrosequencing for community 16S rRNA analysis. We observe a 2–4 fold reduction in the number of observed OTUs (operational taxonomic units) comparing denoised with non-denoised data. ~50,000 sequences can be denoised on a laptop within an hour, two orders of magnitude faster than published techniques. We demonstrate the effects of denoising on alpha and beta diversity of large 16S rRNA datasets.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Gastrointestinal Tract / microbiology
  • Humans
  • Metagenome / genetics*
  • Sequence Analysis, DNA / methods*