Mass spectrometry in high-throughput proteomics: ready for the big time

Nat Methods. 2010 Sep;7(9):681-5. doi: 10.1038/nmeth0910-681.


Mass spectrometry has evolved and matured to a level where it is able to assess the complexity of the human proteome. We discuss some of the expected challenges ahead and promising strategies for success.

MeSH terms

  • Databases, Protein
  • Humans
  • Mass Spectrometry / trends*
  • Proteome / analysis*
  • Proteomics / methods*
  • Quality Control
  • Reproducibility of Results
  • Sensitivity and Specificity


  • Proteome