The glucanase-soluble mannoproteins limit cell wall porosity in Saccharomyces cerevisiae

Yeast. Nov-Dec 1990;6(6):491-9. doi: 10.1002/yea.320060606.

Abstract

The cell wall porosity of batch-grown Saccharomyces cerevisiae was maximal in the early exponential phase and fell off rapidly to lower levels in later growth phases. Treatment of stationary-phase cells with alpha-mannosidase restored wall porosity to the level of cells in early exponential phase. When cells in the early exponential phase were treated with alpha-mannosidase, or tunicamycin, an inhibitor of N-glycosylation, even higher porosities were obtained. Mutants with truncated mannan side-chains in their wall proteins also had very porous walls. The importance of the mannan side-chains for wall porosity was also seen during sexual induction. Treatment with alpha pheromone, which leads to the formation of wall proteins with shorter mannan side-chains, enhanced wall porosity. Disulphide bridges also affect cell wall porosity. They were predominantly found in the glucanase-soluble wall proteins. Because the main part of the mannan side-chains is also found in this family of wall proteins, our results demonstrate that the glucanase-soluble mannoproteins limit cell wall porosity in yeast.

MeSH terms

  • Benzenesulfonates / pharmacology
  • Cell Wall / drug effects
  • Cell Wall / metabolism
  • Cell Wall / ultrastructure
  • DEAE-Dextran / pharmacology
  • Disulfides / metabolism
  • Hydrolases / pharmacology
  • Mannosidases / metabolism*
  • Membrane Glycoproteins / metabolism*
  • Porosity
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae / ultrastructure*
  • Tunicamycin / metabolism*
  • alpha-Mannosidase

Substances

  • Benzenesulfonates
  • Disulfides
  • Membrane Glycoproteins
  • mannoproteins
  • Tunicamycin
  • zymolyase
  • C.I. Fluorescent Brightening Agent 28
  • DEAE-Dextran
  • Hydrolases
  • Mannosidases
  • alpha-Mannosidase