Detection of metallo-β-lactamase genes in clinical specimens by a commercial multiplex PCR system

J Microbiol Methods. 2010 Nov;83(2):185-7. doi: 10.1016/j.mimet.2010.08.014. Epub 2010 Aug 31.


hyplex®-MBL ID Multiplex PCR-ELISA, a novel method for identifying metallo-β-lactamase genes directly in clinical specimens, was evaluated using a consecutive collection of 326 samples from three hospitals in Greece characterized by high prevalence of VIM producers. The method exhibited high sensitivity (98.0%) and specificity (98.6%) and was proven reliable in detecting bla(VIM) genes in blood, urine, pus, and sputum samples that, as confirmed by conventional methods, contained various VIM-producing species. Future multicenter studies should be considered for the thorough evaluation of this method and its potential diagnostic utility.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Gram-Negative Bacteria / enzymology*
  • Gram-Negative Bacteria / genetics
  • Gram-Negative Bacteria / isolation & purification
  • Gram-Negative Bacterial Infections / microbiology*
  • Greece
  • Humans
  • Microbial Sensitivity Tests / methods
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • beta-Lactamases / biosynthesis
  • beta-Lactamases / genetics*


  • Bacterial Proteins
  • beta-Lactamases