Further diversity of the Staphylococcus intermedius group and heterogeneity in the MboI restriction site used for Staphylococcus pseudintermedius species identification

J Vet Diagn Invest. 2010 Sep;22(5):756-9. doi: 10.1177/104063871002200517.


Species identification of 200 beta-hemolysin-producing canine staphylococcal isolates was performed using a recently described polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method (based on MboI restriction of a pta gene fragment), supplemented with biochemical testing and sequencing of housekeeping genes. The PCR-RFLP method misclassified a small fraction (approximately 1%) of the Staphylococcus pseudintermedius population as a result of heterogeneity in the MboI restriction site. A potentially novel species within the Staphylococcus intermedius group (SIG) was found, having closest similarity to S. intermedius based on sequence comparison to the genes sodA, pta, hsp60, tuf, and full-length 16S ribosomal DNA, thus demonstrating further species diversity within the SIG.

MeSH terms

  • Animals
  • Base Sequence
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Dog Diseases / microbiology
  • Dogs
  • Humans
  • Methicillin Resistance
  • Phylogeny
  • Polymerase Chain Reaction / methods
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single Nucleotide
  • RNA, Ribosomal, 16S / genetics
  • Restriction Mapping / methods
  • Sequence Alignment
  • Staphylococcal Infections / epidemiology
  • Staphylococcal Infections / transmission
  • Staphylococcal Infections / veterinary
  • Staphylococcus / classification
  • Staphylococcus / genetics*
  • Staphylococcus aureus / genetics


  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S