Quality Assessment of Platelet Concentrates Prepared by Platelet Rich Plasma-Platelet Concentrate, Buffy Coat Poor-Platelet Concentrate (BC-PC) and apheresis-PC Methods

Asian J Transfus Sci. 2009 Jul;3(2):86-94. doi: 10.4103/0973-6247.53882.

Abstract

Background: Platelet rich plasma-platelet concentrate (PRP-PC), buffy coat poor-platelet concentrate (BC-PC), and apheresis-PC were prepared and their quality parameters were assessed.

Study design: IN THIS STUDY, THE FOLLOWING PLATELET PRODUCTS WERE PREPARED: from random donor platelets (i) platelet rich plasma - platelet concentrate (PRP-PC), and (ii) buffy coat poor-platelet concentrate (BC-PC) and (iii) single donor platelets (apheresis-PC) by different methods. Their quality was assessed using the following parameters: swirling, volume of the platelet concentrate, platelet count, WBC count and pH.

Results: A total of 146 platelet concentrates (64 of PRP-PC, 62 of BC-PC and 20 of apheresis-PC) were enrolled in this study. The mean volume of PRP-PC, BC-PC and apheresis-PC was 62.30+/-22.68 ml, 68.81+/-22.95 ml and 214.05+/-9.91 ml and ranged from 22-135 ml, 32-133 ml and 200-251 ml respectively. The mean platelet count of PRP-PC, BC-PC and apheresis-PC was 7.6+/-2.97 x 1010/unit, 7.3+/-2.98 x 1010/unit and 4.13+/-1.32 x 1011/unit and ranged from 3.2 -16.2 x 1010/unit, 0.6-16.4 x 1010/unit and 1.22-8.9 x 1011/unit respectively. The mean WBC count in PRP-PC (n = 10), BC-PC (n = 10) and apheresis-PC (n = 6) units was 4.05+/-0.48 x 107/unit, 2.08+/-0.39 x 107/unit and 4.8+/-0.8 x 106/unit and ranged from 3.4 -4.77 x 107/unit, 1.6-2.7 x 107/unit and 3.2 - 5.2 x 106/unit respectively. A total of 26 units were analyzed for pH changes. Out of these units, 10 each were PRP-PC and BC-PC and 6 units were apheresis-PC. Their mean pH was 6.7+/-0.26 (mean+/-SD) and ranged from 6.5 - 7.0 and no difference was observed among all three types of platelet concentrate.

Conclusion: PRP-PC and BC-PC units were comparable in terms of swirling, platelet count per unit and pH. As expected, we found WBC contamination to be less in BC-PC than PRP-PC units. Variation in volume was more in BC-PC than PRP-PC units and this suggests that further standardization is required for preparation of BC-PC. As compared to the above two platelet concentrates, all the units of apheresis-PC fulfilled the desired quality control criteria of volume. Apheresis-PC units showed better swirling and platelet count than PRP-PCs and BC-PCs. All the platelet concentrates units had pH well above the recommended norm.

Keywords: Corrected count increment; buffy coat poor-platelet concentrate; percentage recovery; platelet concentrate; platelet rich plasma-platelet concentrate; random donor platelet; single donor platelets.