P21-activated protein kinase 1 induces colorectal cancer metastasis involving ERK activation and phosphorylation of FAK at Ser-910

Int J Oncol. 2010 Oct;37(4):951-62.


Pak1 has been reported to be overexpressed in colorectal cancer, but the role of Pak1 in colorectal cancer remains unclear. In this study, Pak1 expression and activity were associated with aggressive behavior of colorectal cancer. Overexpression of Pak1 increased colorectal cancer cell motility and invasion, whereas down-regulation of Pak1 expression or activity reduced colorectal cancer cell migration and invasion. In addition, activated Pak1 inhibited stress fiber and focal adhesion complex formation in colorectal cancer cells and led to formation of motile phenotypes. Importantly, activated Pak1 elicited phosphorylation of FAK at Ser-910 via an ERK-dependent pathway in colorectal cancer cell lines and clinical samples. In conclusion, our results suggest that activated Pak1 regulates colorectal cancer metastasis requiring an ERK-dependent phosphorylation of FAK at Ser-910.

MeSH terms

  • Cell Line, Tumor
  • Cell Movement*
  • Cell Proliferation
  • Colorectal Neoplasms / enzymology*
  • Colorectal Neoplasms / genetics
  • Colorectal Neoplasms / secondary
  • Enzyme Activation
  • Female
  • Focal Adhesion Kinase 1 / metabolism*
  • Focal Adhesions / enzymology
  • Humans
  • Male
  • Middle Aged
  • Mitogen-Activated Protein Kinase 1 / metabolism*
  • Mitogen-Activated Protein Kinase 3 / metabolism*
  • Neoplasm Invasiveness
  • Neoplasm Staging
  • Phenotype
  • Phosphorylation
  • RNA Interference
  • Serine
  • Stress Fibers / enzymology
  • Transfection
  • p21-Activated Kinases / genetics
  • p21-Activated Kinases / metabolism*


  • Serine
  • Focal Adhesion Kinase 1
  • PTK2 protein, human
  • PAK1 protein, human
  • p21-Activated Kinases
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase 3