Cholesterol intake modulates plasma triglyceride levels in glycosylphosphatidylinositol HDL-binding protein 1-deficient mice

Arterioscler Thromb Vasc Biol. 2010 Nov;30(11):2106-13. doi: 10.1161/ATVBAHA.110.214403. Epub 2010 Sep 2.


Objective: To determine whether plasma triglyceride levels in adult Glycosylphosphatidylinositol HDL-binding protein 1 (GPIHBP1)-deficient (Gpihbp1(-/-)) mice would be sensitive to cholesterol intake.

Methods and results: Gpihbp1(-/-) mice were fed a Western diet containing 0.15% cholesterol. After 4 to 8 weeks, their plasma triglyceride levels were 113 to 135 mmol/L. When 0.005% ezetimibe was added to the diet to block cholesterol absorption, Lpl expression in the liver was reduced significantly, and the plasma triglyceride levels were significantly higher (>170 mmol/L). We also assessed plasma triglyceride levels in Gpihbp1(-/-) mice fed Western diets containing either high (1.3%) or low (0.05%) amounts of cholesterol. The high-cholesterol diet significantly increased Lpl expression in the liver and lowered plasma triglyceride levels.

Conclusions: Treatment of Gpihbp1(-/-) mice with ezetimibe lowers Lpl expression in the liver and increases plasma triglyceride levels. A high-cholesterol diet had the opposite effects. Thus, cholesterol intake modulates plasma triglyceride levels in Gpihbp1(-/-) mice.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anticholesteremic Agents / pharmacology*
  • Azetidines / pharmacology*
  • Cholesterol / metabolism*
  • Dietary Fats / metabolism*
  • Disease Models, Animal
  • Ezetimibe
  • Liver / drug effects
  • Liver / metabolism
  • Mice
  • Receptors, Lipoprotein / deficiency*
  • Triglycerides / blood
  • Triglycerides / metabolism*


  • Anticholesteremic Agents
  • Azetidines
  • Dietary Fats
  • GPI-HBP1 protein, mouse
  • Receptors, Lipoprotein
  • Triglycerides
  • Cholesterol
  • Ezetimibe