Transcript analysis of stem cells

Methods Enzymol. 2010;479:73-91. doi: 10.1016/S0076-6879(10)79004-2.

Abstract

Quantitative real-time polymerase chain reaction (qRT-PCR) is a flexible and scalable method for analyzing transcript abundance that can be used at a single gene or high-throughput (>100 genes) level. Information obtained from this technique can be used as an indicator of potential regulation of glycosylation at the transcript level when combined with glycan structural or protein abundance data. This chapter describes detailed methods to design and perform qRT-PCR analyses and provides examples of information that can be obtained from the technique.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Differentiation
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Gene Transfer Techniques
  • Genetic Markers
  • Humans
  • Mice
  • Polysaccharides / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stem Cells*

Substances

  • Genetic Markers
  • Polysaccharides