Prestressed nuclear organization in living cells

Methods Cell Biol. 2010;98:221-39. doi: 10.1016/S0091-679X(10)98010-2.

Abstract

The nucleus is maintained in a prestressed state within eukaryotic cells, stabilized mechanically by chromatin structure and other nuclear components on its inside, and cytoskeletal components on its outside. Nuclear architecture is emerging to be critical to the governance of chromatin assembly, regulation of genome function and cellular homeostasis. Elucidating the prestressed organization of the nucleus is thus important to understand how the nuclear architecture impinges on its function. In this chapter, various chemical and mechanical methods have been described to probe the prestressed organization of the nucleus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Biomechanical Phenomena / drug effects
  • Biomechanical Phenomena / genetics
  • Biomechanical Phenomena / physiology
  • Biomechanical Phenomena / radiation effects
  • Cell Nucleus / genetics*
  • Cell Nucleus / physiology*
  • Cell Nucleus / ultrastructure
  • Cells / cytology*
  • Cells / drug effects
  • Cells / metabolism
  • Cells / radiation effects
  • Chromatin Assembly and Disassembly / drug effects
  • Chromatin Assembly and Disassembly / physiology*
  • Chromatin Assembly and Disassembly / radiation effects
  • Cytoskeleton / chemistry
  • Cytoskeleton / drug effects
  • Cytoskeleton / metabolism
  • Cytoskeleton / radiation effects
  • Enzyme Inhibitors / pharmacology
  • Humans
  • Lasers
  • Models, Biological
  • Photobleaching
  • Stress, Mechanical

Substances

  • Enzyme Inhibitors