Applications of heparin and heparan sulfate microarrays

Methods Enzymol. 2010;478:197-218. doi: 10.1016/S0076-6879(10)78009-5.

Abstract

Carbohydrate microarrays have become crucial tools for revealing the biological interactions and functions of glycans, primarily because the microarray format enables the investigation of large numbers of carbohydrates at a time. Heparan sulfate (HS) and heparin are the most structurally complex glycosaminoglycans (GAGs). In this chapter, we describe the preparation of a small library of HS/heparin oligosaccharides, and the fabrication of HS/heparin microarrays that have been used to establish HS/heparin-binding profiles. Fibroblast growth factors (FGFs), natural cytotoxicity receptors (NCRs), and chemokines were screened to illuminate the very important biological functions of these glycans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbohydrate Sequence
  • Heparin / chemistry*
  • Heparitin Sulfate / chemistry*
  • Humans
  • Microarray Analysis* / instrumentation
  • Microarray Analysis* / methods
  • Molecular Sequence Data

Substances

  • Heparin
  • Heparitin Sulfate