17-AAG increases autophagic removal of mutant androgen receptor in spinal and bulbar muscular atrophy

Neurobiol Dis. 2011 Jan;41(1):83-95. doi: 10.1016/j.nbd.2010.08.023. Epub 2010 Sep 9.


Several types of motorneuron diseases are linked to neurotoxic mutant proteins. These acquire aberrant conformations (misfolding) that trigger deleterious downstream events responsible for neuronal dysfunction and degeneration. The pharmacological removal of misfolded proteins might thus be useful in these diseases. We utilized a peculiar motorneuronal disease model, spinobulbar muscular atrophy (SBMA), in which the neurotoxicity of the protein involved, the mutant androgen receptor (ARpolyQ), can be modulated by its ligand testosterone (T). 17-(allylamino)-17-demethoxygeldanamycin (17-AAG) has already been proven to exert beneficial action in SBMA. Here we demonstrated that 17-AAG exerts its pro-degradative activity on mutant ARpolyQ without impacting on proteasome functions. 17-AAG removes ARpolyQ misfolded species and aggregates by activating the autophagic system. We next analyzed the 17-AAG effects on two proteins (SOD1 and TDP-43) involved in related motorneuronal diseases, such as amyotrophic lateral sclerosis (ALS). In these models 17-AAG was unable to counteract protein aggregation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autophagy / drug effects*
  • Autophagy / physiology
  • Benzoquinones / pharmacology*
  • Benzoquinones / therapeutic use
  • Cell Line, Transformed
  • Lactams, Macrocyclic / pharmacology*
  • Lactams, Macrocyclic / therapeutic use
  • Mice
  • Motor Neurons / drug effects*
  • Motor Neurons / metabolism
  • Motor Neurons / pathology
  • Muscular Atrophy, Spinal / drug therapy*
  • Muscular Atrophy, Spinal / metabolism
  • Muscular Atrophy, Spinal / pathology
  • Mutation / genetics
  • Neuroprotective Agents / pharmacology
  • Proteostasis Deficiencies / drug therapy*
  • Proteostasis Deficiencies / metabolism
  • Proteostasis Deficiencies / pathology
  • Receptors, Androgen / genetics
  • Receptors, Androgen / metabolism*
  • Up-Regulation / drug effects*
  • Up-Regulation / physiology


  • Benzoquinones
  • Lactams, Macrocyclic
  • Neuroprotective Agents
  • Receptors, Androgen
  • tanespimycin