Expression of candidate markers for stem/progenitor cells in the inner ears of developing and adult GFAP and nestin promoter-GFP transgenic mice

Gene Expr Patterns. Jan-Feb 2011;11(1-2):22-32. doi: 10.1016/j.gep.2010.08.008. Epub 2010 Sep 9.

Abstract

Loss of hair cells in the mammalian cochlea leads to permanent sensori-neural hearing loss. Hair cells degenerate and their places are taken by phalangeal scars formed by non-sensory supporting cells. Current data indicate that early postnatal post-mitotic supporting cells can proliferate and differentiate into hair cell-like cells in culture. In this study, we used GFAP and nestin promoter-GFP transgenic mice in combination with other stem cell markers to characterize supporting cell subtypes in the postnatal day-3 (P3) and adult organs of Corti with potential stem/progenitor cell phenotype. In P3 organ of Corti, we show GFAP-GFP signal in all the supporting cell subtypes while the nestin-GFP was restricted to the supporting cells in the inner hair cell area. At this stage, GFAP and selected stem/progenitor markers displayed overlapping expression pattern in the supporting cell population. In the adult, GFAP expression is down-regulated from the supporting cells in the outer hair cell area and nestin expression is down-regulated in the supporting cells of the inner hair cell area. Sox2 and Jagged1 expression is maintained in the mature supporting cells, while Abcg2 was down-regulated in these cells. In contrast, GFAP and Abcg2 expression was up-regulated in the inner sulcus limbal cells outside the mature organ of Corti's area. Using quantitative reverse transcription-PCR, we found a decrease in transcripts for Jagged1 and Sox2 in adult cochleae. Our findings suggest that the loss of regenerative capacity of the adult organ of Corti is related to down-regulation of stem/progenitor key-markers from the mature supporting cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers
  • Cochlea / cytology*
  • Gene Expression*
  • Green Fluorescent Proteins / analysis
  • Green Fluorescent Proteins / genetics
  • Hair Cells, Auditory, Inner / cytology*
  • Hair Cells, Auditory, Inner / metabolism
  • Intermediate Filament Proteins / analysis
  • Intermediate Filament Proteins / genetics
  • Mice
  • Mice, Transgenic
  • Nerve Tissue Proteins / analysis
  • Nerve Tissue Proteins / genetics
  • Nestin
  • Organ of Corti / cytology
  • Stem Cells / cytology*

Substances

  • Biomarkers
  • Intermediate Filament Proteins
  • Nerve Tissue Proteins
  • Nes protein, mouse
  • Nestin
  • Green Fluorescent Proteins